Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. The powerful hands-on methods collected here will help even the novice to produce first-class cover-quality confocal images.
An Introduction to Confocal Imaging.- Practical Considerations for Collecting Confocal Images.- Fluorescent Probes for Confocal Microscopy.- Imaging Gene Expression Using Antibody Probes.- Single and Double FISH Protocols for Drosophila.- Confocal Microscopy of Plant Cells.- Preparation of Yeast Cells for Confocal Microscopy.- Confocal Methods for Caenorhabditis elegans.- Imaging Sea Urchin Fertilization.- Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy.- Confocal Microscopy on Xenopus laevis Oocytes and Embryos.- Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos.- Confocal Imaging of Living Cells in Intact Embryos.- Live Confocal Analysis with Fluorescently Labeled Proteins.- Live Imaging with Green Fluorescent Protein.- Fluorescent Calcium Indicators:Subcellular Behavior and Use in Confocal Imaging.- Intracellular pH and pCa Measurement.- Measuring Dynamic Cell Volume In Situ by Confocal Microscopy.- Imaging Thick Tissues with Confocal Microscopy.- Measurement in the Confocal Microscope.- Presentation of Confocal Images.- The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides.- Information Management of Confocal Microscopy Images.- Morphing Confocal Images and Digital Movie Production.
"This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images. . . Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided. . . In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended."-Quarterly Review of Biology
"...almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and protocols for confocal microscopy is therefore very timely...The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money."-Cell Biology International
" This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes...there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively."-Microbiology Today
"...The texts are aimed at the biological user...for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations..."-Ultramicroscopy
"...excellent information as an introductory basis for confocal microscopy."-Cellular and Molecular Biology
